pgl3-basic firefly luciferase expression vector (Promega)
90
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Promega
pgl3-basic firefly luciferase expression vector
Pgl3 Basic Firefly Luciferase Expression Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3-basic+firefly+luciferase+expression+vector/pgl3+basic/pm36293554-293-16-21
Average 90 stars, based on 1 article reviews
Pgl3 Basic Firefly Luciferase Expression Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3-basic+firefly+luciferase+expression+vector/pgl3+basic/pm36293554-293-16-21
Average 90 stars, based on 1 article reviews
pgl3-basic firefly luciferase expression vector - by Bioz Stars,
2026-09
90/100 stars
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other:Article Title: An epigenetic mechanism regulates germ cell-specific expression of the porcine Deleted in Azoospermia-Like (DAZL) gene. Article Snippet: The Deleted in Azoospermia-Like (DAZL) gene is specifically expressed in fetal and adult gonads.. While DAZL is known to play a role during gametogenesis, the mechanisms governing its germ cell-specific expression remain unclear.. We identified the 50 untranslated region (UTR) of the porcine DAZL gene and cloned and characterized 2 kilobase pairs of its TATA-less 50 flanking region, identifying CpG-rich regions within the proximal promoter. Article Title: The role of prostaglandin E receptor-dependent signaling via cAMP in Mdr1b gene activation in primary rat hepatocyte cultures. Article Snippet: Multidrug resistance (mdr) proteins of the mdr1 type function as multispecific xenobiotic transporters in hepatocytes.. In the liver, mdr1 overexpression occurs during regeneration, cirrhosis, and hepatocarcinogenesis and may contribute to primary chemotherapy resistance.. Cultured rat hepatocytes exhibit a time-dependent “intrinsic” increase in functional mdr1b expression, which depends on cyclooxygenase-catalyzed prostaglandin E2 release. Article Title: Sp1 and Sp3 mediate basal and serum-induced expression of human CENP-W. Article Snippet: Cancer-upregulated gene 2 (CUG2), which was named since it was originally identified as a putative oncogene up-regulated in various human cancers, was recently renamed CENP-W based on the new findings that it is a component of the centromeric complex playing a crucial role in the assembly of functional kinetochore complex during mitosis.. To understand the transcriptional regulation of CENP-W, we analyzed its TATA-less promoter and identified a GC-rich putative Sp1 binding site located at -46 to -36 that was critical in CENP-W expression.. Competitive electrophoretic gel mobility shift assay using mutated oligos and supershift assays with Sp1 and Sp3 antibodies demonstrated that both proteins specifically bound to this promoter region. Article Title: A Functional Polymorphism in PIN1 that Prevents its Suppression by AP4 Is Associated with Delayed Onset of Alzheimer's disease Article Snippet: The fragment was cloned into the previously generated Pin1 promoter construct (−2300 to +1) ( Ryo et al. 2002 ) with Article Title: Identification and Expression Analysis of Dsx and Its Positive Transcriptional Regulation of IAG in Black Tiger Shrimp ( Penaeus monodon ). Article Snippet: The vectors were named pGL3-basicLuc1, pGL3-basic-Luc2, pGL3-basic-Luc3, and pGL3-basic-Luc4, and each fragment was cloned into the Clone Assay:Article Title: Stearoyl-CoA desaturase 1 expression is downregulated in liver and udder during E. coli mastitis through enhanced expression of repressive C/EBP factors and reduced expression of the inducer SREBP1A Article Snippet: PCR products were first cloned into pGEM-Teasy (Promega), validated by sequencing and the inserts were then excised via the artificially introduced restriction sites. .. They were subsequently cloned into the promoter-less pGL3-basic Luciferase:Article Title: Stearoyl-CoA desaturase 1 expression is downregulated in liver and udder during E. coli mastitis through enhanced expression of repressive C/EBP factors and reduced expression of the inducer SREBP1A Article Snippet: PCR products were first cloned into pGEM-Teasy (Promega), validated by sequencing and the inserts were then excised via the artificially introduced restriction sites. .. They were subsequently cloned into the promoter-less pGL3-basic Article Title: Expression of STK11 gene and its promoter activity in MCF control and cancer cells Article Snippet: .. Cloning The STK11 promoter reporter construct was generated by amplifying a 3995 bp fragment of the STK11 promoter using primers STK11prom-XhoI-F 5′ CGGGAAT CTCGAG TTGGAAATTCAGTG TGTAGGGCA 3′ and STK11prom-HindIII-R 5′ AAAGCGC AAGCTT CAACAAAAACCCCA AAAGGA 3′ resulting in amplification of a product containing XhoI and HindIII restriction enzyme cleavage sites to clone into pGL3-basic Article Title: Expression of STK11 gene and its promoter activity in MCF control and cancer cells Article Snippet: .. The STK11 promoter reporter construct was generated by amplifying a 3995 bp fragment of the STK11 promoter using primers STK11prom-XhoI-F 5′ CGGGAAT CTCGAG TTGGAAATTCAGTG TGTAGGGCA 3′ and STK11prom-HindIII-R 5′ AAAGCGC AAGCTT CAACAAAAACCCCA AAAGGA 3′ resulting in amplification of a product containing XhoI and HindIII restriction enzyme cleavage sites to clone into pGL3-basic Expressing:Article Title: Stearoyl-CoA desaturase 1 expression is downregulated in liver and udder during E. coli mastitis through enhanced expression of repressive C/EBP factors and reduced expression of the inducer SREBP1A Article Snippet: PCR products were first cloned into pGEM-Teasy (Promega), validated by sequencing and the inserts were then excised via the artificially introduced restriction sites. .. They were subsequently cloned into the promoter-less pGL3-basic Article Title: Expression of STK11 gene and its promoter activity in MCF control and cancer cells Article Snippet: .. Cloning The STK11 promoter reporter construct was generated by amplifying a 3995 bp fragment of the STK11 promoter using primers STK11prom-XhoI-F 5′ CGGGAAT CTCGAG TTGGAAATTCAGTG TGTAGGGCA 3′ and STK11prom-HindIII-R 5′ AAAGCGC AAGCTT CAACAAAAACCCCA AAAGGA 3′ resulting in amplification of a product containing XhoI and HindIII restriction enzyme cleavage sites to clone into pGL3-basic Article Title: Expression of STK11 gene and its promoter activity in MCF control and cancer cells Article Snippet: .. The STK11 promoter reporter construct was generated by amplifying a 3995 bp fragment of the STK11 promoter using primers STK11prom-XhoI-F 5′ CGGGAAT CTCGAG TTGGAAATTCAGTG TGTAGGGCA 3′ and STK11prom-HindIII-R 5′ AAAGCGC AAGCTT CAACAAAAACCCCA AAAGGA 3′ resulting in amplification of a product containing XhoI and HindIII restriction enzyme cleavage sites to clone into pGL3-basic Plasmid Preparation:Article Title: Stearoyl-CoA desaturase 1 expression is downregulated in liver and udder during E. coli mastitis through enhanced expression of repressive C/EBP factors and reduced expression of the inducer SREBP1A Article Snippet: PCR products were first cloned into pGEM-Teasy (Promega), validated by sequencing and the inserts were then excised via the artificially introduced restriction sites. .. They were subsequently cloned into the promoter-less pGL3-basic Article Title: Expression of STK11 gene and its promoter activity in MCF control and cancer cells Article Snippet: .. Cloning The STK11 promoter reporter construct was generated by amplifying a 3995 bp fragment of the STK11 promoter using primers STK11prom-XhoI-F 5′ CGGGAAT CTCGAG TTGGAAATTCAGTG TGTAGGGCA 3′ and STK11prom-HindIII-R 5′ AAAGCGC AAGCTT CAACAAAAACCCCA AAAGGA 3′ resulting in amplification of a product containing XhoI and HindIII restriction enzyme cleavage sites to clone into pGL3-basic Article Title: Expression of STK11 gene and its promoter activity in MCF control and cancer cells Article Snippet: .. The STK11 promoter reporter construct was generated by amplifying a 3995 bp fragment of the STK11 promoter using primers STK11prom-XhoI-F 5′ CGGGAAT CTCGAG TTGGAAATTCAGTG TGTAGGGCA 3′ and STK11prom-HindIII-R 5′ AAAGCGC AAGCTT CAACAAAAACCCCA AAAGGA 3′ resulting in amplification of a product containing XhoI and HindIII restriction enzyme cleavage sites to clone into pGL3-basic Cloning:Article Title: Expression of STK11 gene and its promoter activity in MCF control and cancer cells Article Snippet: .. Cloning The STK11 promoter reporter construct was generated by amplifying a 3995 bp fragment of the STK11 promoter using primers STK11prom-XhoI-F 5′ CGGGAAT CTCGAG TTGGAAATTCAGTG TGTAGGGCA 3′ and STK11prom-HindIII-R 5′ AAAGCGC AAGCTT CAACAAAAACCCCA AAAGGA 3′ resulting in amplification of a product containing XhoI and HindIII restriction enzyme cleavage sites to clone into pGL3-basic Construct:Article Title: Expression of STK11 gene and its promoter activity in MCF control and cancer cells Article Snippet: .. Cloning The STK11 promoter reporter construct was generated by amplifying a 3995 bp fragment of the STK11 promoter using primers STK11prom-XhoI-F 5′ CGGGAAT CTCGAG TTGGAAATTCAGTG TGTAGGGCA 3′ and STK11prom-HindIII-R 5′ AAAGCGC AAGCTT CAACAAAAACCCCA AAAGGA 3′ resulting in amplification of a product containing XhoI and HindIII restriction enzyme cleavage sites to clone into pGL3-basic Article Title: Expression of STK11 gene and its promoter activity in MCF control and cancer cells Article Snippet: .. The STK11 promoter reporter construct was generated by amplifying a 3995 bp fragment of the STK11 promoter using primers STK11prom-XhoI-F 5′ CGGGAAT CTCGAG TTGGAAATTCAGTG TGTAGGGCA 3′ and STK11prom-HindIII-R 5′ AAAGCGC AAGCTT CAACAAAAACCCCA AAAGGA 3′ resulting in amplification of a product containing XhoI and HindIII restriction enzyme cleavage sites to clone into pGL3-basic Generated:Article Title: Expression of STK11 gene and its promoter activity in MCF control and cancer cells Article Snippet: .. Cloning The STK11 promoter reporter construct was generated by amplifying a 3995 bp fragment of the STK11 promoter using primers STK11prom-XhoI-F 5′ CGGGAAT CTCGAG TTGGAAATTCAGTG TGTAGGGCA 3′ and STK11prom-HindIII-R 5′ AAAGCGC AAGCTT CAACAAAAACCCCA AAAGGA 3′ resulting in amplification of a product containing XhoI and HindIII restriction enzyme cleavage sites to clone into pGL3-basic Article Title: Expression of STK11 gene and its promoter activity in MCF control and cancer cells Article Snippet: .. The STK11 promoter reporter construct was generated by amplifying a 3995 bp fragment of the STK11 promoter using primers STK11prom-XhoI-F 5′ CGGGAAT CTCGAG TTGGAAATTCAGTG TGTAGGGCA 3′ and STK11prom-HindIII-R 5′ AAAGCGC AAGCTT CAACAAAAACCCCA AAAGGA 3′ resulting in amplification of a product containing XhoI and HindIII restriction enzyme cleavage sites to clone into pGL3-basic Amplification:Article Title: Expression of STK11 gene and its promoter activity in MCF control and cancer cells Article Snippet: .. Cloning The STK11 promoter reporter construct was generated by amplifying a 3995 bp fragment of the STK11 promoter using primers STK11prom-XhoI-F 5′ CGGGAAT CTCGAG TTGGAAATTCAGTG TGTAGGGCA 3′ and STK11prom-HindIII-R 5′ AAAGCGC AAGCTT CAACAAAAACCCCA AAAGGA 3′ resulting in amplification of a product containing XhoI and HindIII restriction enzyme cleavage sites to clone into pGL3-basic Article Title: Expression of STK11 gene and its promoter activity in MCF control and cancer cells Article Snippet: .. The STK11 promoter reporter construct was generated by amplifying a 3995 bp fragment of the STK11 promoter using primers STK11prom-XhoI-F 5′ CGGGAAT CTCGAG TTGGAAATTCAGTG TGTAGGGCA 3′ and STK11prom-HindIII-R 5′ AAAGCGC AAGCTT CAACAAAAACCCCA AAAGGA 3′ resulting in amplification of a product containing XhoI and HindIII restriction enzyme cleavage sites to clone into pGL3-basic |
